GEWS

GEWS: Gene Expression Web Services

Functionality from Prep, Engene and the quantile normalization implementation has been provided as ACGT and BioMoby compliant web-services and registered in the different metadata repository.

PRODUCT DESCRIPTION

Data analysis in bioinformatics is typically delegated to external web-services which are designed to support interoperable machine-to-machine interaction over a network.

Two main platforms are common in gene expression analysis: two-channel (competitive) and one-channel analysis hybridization. Two-channel microarrays are typically hybridized with cDNA prepared from two samples to be compared at the same time (e.g. diseased versus healthy samples), while one-channel (typically Affymetrix) contains a set of probes for genes (technical replications) from which probe-set values are obtained to represent the gene expression. Several analysis methods can be applied to ensure data quality, spot filtration, inter- and intra-slide normalization, replicate resolution, dye-swapping, random error removal and statistical analyses (see Prep+07 ).

Once data is normalized, it can be used to generate a gene expression matrix. At this stage, more complex data analysis tools can be applied. One example is Engene , which is useful tool for storing, visualizing and processing large sets of expression patterns. In the case of one-channel data, there are several alternatives to normalization of cell data (for example, the Bioconductor R packages Affy (www.bioconductor.org>).

Functionality from Prep, Engene and the quantile normalization implementation has been provided as ACGT and BioMoby compliant web-services and registered in the different metadata repository.

SCREENSHOTS

Uso de cookies

Este sitio web utiliza cookies para que usted tenga la mejor experiencia de usuario. Si continúa navegando está dando su consentimiento para la aceptación de las mencionadas cookies y la aceptación de nuestra política de cookies, pinche el enlace para mayor información.plugin cookies

ACEPTAR
Aviso de cookies